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Committee for collaboration of Czech Republic

with Joint Institute for Nuclear Research in Dubna Login
Formation of Direct and Enzymatic DNA Double-Strand Breaks in the Presence of Repair Inhibitors after Exposure to Radiations of Different Quality

Author
Chausov V.N. JINR
Ježková  Lucie, Ing., Ph.D. Istitute of Chemical Technology Prague
et al.  different institutions

Year
2018

Scientific journal
Physics of Particles and Nuclei Letters, 15 (6), 700-710

Web


Abstract
With the use of the DNA comet assay and immunocytochemical staining, regularities have been studied in the induction and repair of DNA double-strand breaks(DSBs) in human cells after exposure to Co-60 -rays and accelerated heavy ions with different linear energy transfer (LET) in the presence of the DNA repair inhibitors cytosine arabinoside and hydroxyurea. It is shown that for heavy ions the agents' modifying effect decreases with increasing particles' LET. The approach involving DNA synthesis inhibitors used in this study allows an estimation of the proportion of enzymatic DNA DSBs in total DSB yield after exposure to ionizing radiations of different quality.

Cite article as:
V. Chausov, L. Ježková , . et al., "Formation of Direct and Enzymatic DNA Double-Strand Breaks in the Presence of Repair Inhibitors after Exposure to Radiations of Different Quality", Physics of Particles and Nuclei Letters, 15 (6), 700-710 (2018)