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Committee for cooperation of the Czech Republic with Joint Institute for Nuclear Research Login
Heavy ion irradiation induced mutations in the HPRT gene of V79 cells.

Author
Bláha Pavel, Ing. Faculty of Nuclear Sciences and Physical Engineering CTU, JINR Dubna
Koshlan I.V. JINR
Koshlan N.A. JINR
Govorun R.D. JINR

Year
2014

Scientific journal
Book of Abstracts. XVIII Conference of Young Scientists and Specialists, p. 87

Web


Abstract
Genomic instability has been observed in remote progeny of Chinese hamster cells (line V79) exposed to two different accelerated heavy ions – 18O and 20Ne. Linear energy transfer (LET) values of these radiations were in the interval 116-153 keV/µm. Characteristics of the dependence of HPRT mutant fraction on expression time has been measured after exposure to doses: 0.5, 1, and 2 Gy. It was found that mutant fraction is rising with the expression time; reaching maximum; and decreasing again. After approximately 40-45 days (80-95 cell generations), mutant fraction has returned back to the level characteristic for spontaneous mutants, under all irradiation conditions. Position of the mutant fraction maximum was moving with different LET.

Ocenění
Third place in the “scientific-research experimental work” category.

Cite article as:
P. Bláha, I. Koshlan, N. Koshlan, R. Govorun, "Heavy ion irradiation induced mutations in the HPRT gene of V79 cells.", Book of Abstracts. XVIII Conference of Young Scientists and Specialists, p. 87 (2014)